In Vitro Fertilization - Innovative Clinical, Lab. Aspects


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Why it's done

All rights reserved. This article has been cited by other articles in PMC. Abstract In vitro fertilization, popularly referred to as IVF, has captured the attention of the public since its sensational introduction in Keywords: IVF, assisted reproduction.

Introduction The birth of Louise Brown in was the culmination of decades of scientific research in reproductive medicine. Open in a separate window.

Introduction

Figure 1. Timeline of major milestones in assisted reproductive technologies. The early days of IVF Prior to , women without functioning fallopian tubes were largely considered to be sterile by their physicians. Oocyte donation While advances in early IVF refined the technology for treating women with tubal disease, those with natural or premature ovarian failure had no effective fertility treatments until Figure 2.

Embryo cryopreservation Clinical and laboratory methodology used for ART continued to evolve and improve, and a surplus of embryos in excess to what is used or needed for the initial IVF treatment became increasingly commonplace. Refinements in technique: moving away from the operating room During the mids, efforts to simplify and improve success rates of assisted reproduction led to the development of gamete intrafallopian transfer GIFT where oocytes were laparoscopically retrieved and immediately transferred into fallopian tubes along with sperm Asch et al Figure 3.

Comparing techniques for embryo transfer Multiple studies in the early s comparing IVF, ZIFT, and GIFT often showed conflicting results due to multiple confounding variables inherent to different patient-selection criteria and the wide variation of practice used from clinic to clinic.

Addressing the needs of infertile men As IVF became more commonplace in the treatment of female infertility, male infertility remained a limiting factor to overall success. IVF and preimplantation genetic diagnosis Prior to , options to prevent transmission of genetic defects were limited to performing chorionic villus sampling or amniocentesis and offering abortion if the fetus were found to be affected. Fertility preservation for cancer patients Contemporary investigative efforts continue to focus on providing even more couples the opportunity to have healthy children.

Conclusion In the three decades following the birth of Louise Brown, innovations in ART have overcome numerous seemingly insurmountable barriers to allow couples the chance to have families. Fetal and maternal morbidity and mortality in menopausal women aged 45—63 years.


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Hum Reprod. Preimplantation genetic diagnosis of inherited cancer: familial adenomatous polyposis coli. J Assist Reprod Genet. Pregnancy after translaparoscopic gamete intrafallopian transfer. A confocal microscopy analysis of the spindle and chromosome configurations of human oocytes cryopreserved at the germinal vesicle and metaphase II stage.

Prenatal testing in ICSI pregnancies: incidence of chromosomal anomalies in karyotypes and relation to sperm parameters. A multi-centre cohort study of the physical health of 5-year-old children conceived after intracytoplasmic sperm injection, in vitro fertilization and natural conception. Accessed on 5 December Use of fluorescence in situ hybridization to assess the chromosomal status of embryos obtained from cryopreserved oocytes.

Fertil Steril. Ovum aspiration diagram [online] Intracytoplasmic sperm injection may increase the risk of imprinting defects. Am J Hum Genet. Fertilising ability of testicular spermatozoa. European Society of Human Reproduction and Embryology. Detection of aneuploidy and chromosomal mosaicism in human embryos during preimplantation sex determination by fluorescent in situ hybridisation, FISH Hum Mol Genet.

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Transvaginal sonographically controlled follicle puncture for oocyte retrieval. Assisted reproductive techniques in patients with Klinefelter syndrome: a critical review. Zygote intrafallopian transfer as a successful treatment for unexplained infertility. Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. Current status of in-vitro fertilisation and implantation of human embryos. Establishing full-term human pregnancies using cleaving embryos grown in vitro. Br J Obstet Gynaecol. Use of third generation gonadotropin-releasing hormone antagonists in in vitro fertilization-embryo transfer: a review.

Obstet Gynecol Surv. The births of five Spanish babies from cryopreserved donated oocytes. Cryopreservation of embryos and ova.

Preimplantation genetic diagnosis

The role of preimplantation diagnosis for aneuploidies. Reprod Biomed Online. Ovarian hyperstimulation syndrome following D-Trp-6 luteinizing hormone-releasing hormone microcapsules and menotropin for in vitro fertilization. Clinical experience with preimplantation diagnosis of sex by dual fluorescent in situ hybridization. Zygote intrafallopian transfer ZIFT : evaluation of 42 cases. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification.

Birth of a normal girl after in vitro fertilization and preimplantation diagnostic testing for cystic fibrosis. N Engl J Med. The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization. The program for in vitro fertilization at Norfolk. Clinical aspects of preimplantation genetic diagnosis for single gene disorders combined with HLA typing.

Culture of preimplantation mouse embryos affects fetal development and the expression of imprinted genes. Biol Reprod. Preimplantation diagnosis of thalassemias. Ultrasonically guided percutaneous aspiration of human follicles under local anesthesia: a new method of collecting oocytes for in vitro fertilization. Collection of human oocytes for in vitro fertilisation by ultrasonically guided follicular puncture. Int J Epidemiol. Increased prevalence of imprinting defects in patients with Angelman syndrome born to subfertile couples. J Med Genet. The establishment and maintenance of pregnancy using in vitro fertilization and embryo donation in a patient with primary ovarian failure.

Partial zona dissection of the human oocyte: a nontraumatic method using micromanipulation to assist zona pellucida penetration.

Penn Fertility Care – Penn Medicine

A prospective controlled study of in-vitro fertilization, gamete intra-fallopian transfer and intrauterine insemination combined with superovulation. Improved implantation after preimplantation genetic diagnosis of aneuploidy. A fast and efficient method for simultaneous X and Y in situ hybridization of human blastomeres. Preimplantation genetic diagnosis.


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Curr Opin Obstet Gynecol. Microinjection of human oocytes: a technique for severe oligoasthenoteratozoospermia. Gonadotrophin therapy for ovulation induction in subfertility associated with polycystic ovary syndrome. Cochrane Database Syst Rev.

IVF And More: 6 Pillars of Medical Tourism in Greece

Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Oocyte retrieval for in-vitro fertilisation by ultrasonically guided needle aspiration via the urethra. Pregnancy in the sixth decade of life: obstetric outcomes in women of advanced reproductive age. Successful pregnancy in a year-old woman. International collaborative study of intracytoplasmic sperm injection-conceived, in vitro fertilization-conceived, and naturally conceived 5-year-old child outcomes: cognitive and motor assessments.

Induction of ovulation for in-vitro fertilisation using buserelin and gonadotropins. Pregnancy after fertilisation with human testicular spermatozoa. Prenat Diagn. By Mahnaz Ashrafi and Kiandokht Kiani. Ortega and Pablo Bosch. Boyer, P. Rodrigues, P.

In Vitro Fertilization - Innovative Clinical, Lab. Aspects In Vitro Fertilization - Innovative Clinical, Lab. Aspects
In Vitro Fertilization - Innovative Clinical, Lab. Aspects In Vitro Fertilization - Innovative Clinical, Lab. Aspects
In Vitro Fertilization - Innovative Clinical, Lab. Aspects In Vitro Fertilization - Innovative Clinical, Lab. Aspects
In Vitro Fertilization - Innovative Clinical, Lab. Aspects In Vitro Fertilization - Innovative Clinical, Lab. Aspects
In Vitro Fertilization - Innovative Clinical, Lab. Aspects In Vitro Fertilization - Innovative Clinical, Lab. Aspects
In Vitro Fertilization - Innovative Clinical, Lab. Aspects In Vitro Fertilization - Innovative Clinical, Lab. Aspects
In Vitro Fertilization - Innovative Clinical, Lab. Aspects In Vitro Fertilization - Innovative Clinical, Lab. Aspects
In Vitro Fertilization - Innovative Clinical, Lab. Aspects In Vitro Fertilization - Innovative Clinical, Lab. Aspects

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