Alias, Journal of Pharmacology and Toxicology, 2: DOI: Full Name:. Online First. Current Issue. Previous Issues. Editorial Board. Submit a Manuscript. Guide to Authors. Subscribe to E-alerts. Comp Biochem Physiol. Pest Biochem Physiol. Pest Sci. Pestic Sci.
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Bull Ent Res. J Biol Chem. Charpentier A, Fournier D: Acetylcholinesterase amount in Drosophila melanogaster in relation to insecticide resistance. PCR Methods Appl.
Biochem Biophys Res Commun. Protein Expr Purif.
Protein Sci. Insect Biochem Mol Biol. Insect Mol Biol. Pestic Biochem Physiol. Nabeshima T, Kozaki T, Tomita T, Kono Y: An amino acid substitution on the second acetylcholinesterase in the pirimicarb-resistant strains of the peach potato aphid, Myzus persicae. Download references. Paris 6 , Philip Daborn Melbourne Univ. Correspondence to Didier Fournier. MAS purified the recombinant proteins and estimated their sensitivity.
AL performed in vitro mutagenesis and production of mutants.
ZHT participated in coordination and DF conceived the study. All authors read and approved the final manuscript. Reprints and Permissions. Search all BMC articles Search. Abstract Background Organophosphate and carbamate insecticides irreversibly inhibit acetylcholinesterase causing death of insects. Results To analyze the most frequent mutations providing insecticide resistance in Drosophila melanogaster acetylcholinesterase, the Ace gene was cloned and sequenced in several strains harvested from different parts of the world.
Conclusion The presence of several alleles in natural populations, offering various resistance to carbamate and organophosphate compounds will complicate the establishment of resistance management programs. Table 1 Point mutations in acetylcholinesterase involved in insecticide resistance. Full size table. Results Point mutations detected in D. Table 2 Alleles found in natural populations of D.
Table 3 Number of each allele found in natural populations of D. Table 4 Geographic distribution of point mutations at positions , , and determined by PASA. Discussion Heterogeneity of populations and insecticide resistance As shown by cDNA sequencing, several alleles coexist in most natural populations. Practical consequences of the combination of mutations involved in insecticide resistance In several cases, single mutations sensitised the enzyme to the insecticide ki ratio was lower than 1.
Acetylcholinesterase: From 3D Structure to Function
Site-directed mutagenesis and protein preparation We generated point mutations using a standard PCR based strategy and verified the identities of individual clones through double stranded plasmid sequencing. Determination of enzyme sensitivity The inhibition mechanism of AChE by organophosphate and carbamate compounds has been described by Aldridge [ 1 ]. References 1. Google Scholar Philippe Menozzi, Ming An Shi contributed equally to this work. Table of contents chapters Table of contents chapters Bert N.
David et al. Pages Ordentlich, Arie et al. Andrew et al.
Ten et al. Pages Pritchard, David. Cholinesterases and Agriculture Pages Wilson, B. Show next xx.
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Related Structure and Function of Cholinesterases and Related Proteins
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